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    Nicola ALESSIO

    Insegnamento di AFP MOLECULAR BIOLOGY

    Corso di laurea magistrale a ciclo unico in MEDICINA E CHIRURGIA

    SSD: BIO/11

    CFU: 1,00

    ORE PER UNITÀ DIDATTICA: 20,00

    Periodo di Erogazione: Primo Semestre

    Italiano

    Lingua di insegnamento

    INGLESE

    English

    Teaching language

    English

    Contents

    Advanced Biomolecular Technologies

    Textbook and course materials

    Molecular Biology Techniques
    A Classroom Laboratory Manual
    4th Edition
    Authors: Sue Carson, Heather B. Miller, Melissa C. Srougi, D. Scott Witherow

    Course objectives

    The student will acquire fundamental knowledge underlying molecular biology techniques.

    Evaluation methods

    Oral examination with a minimum passing grade of 18 and a maximum of 30.

    Course Syllabus

    Agarose Gel Electrophoresis.
    PCR General Principles and Applications.
    RT-PCR, Real-time PCR.
    Restriction Enzymes.
    Types of Restriction-Modification Systems.
    Cloning Basics: Main Types of Plasmids. Selective Markers. Viral Vectors. Chemical Transformation and Electroporation.

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